کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4752103 | 1415992 | 2017 | 11 صفحه PDF | دانلود رایگان |
- Realkalized fed-batch cultures in whey were designed for high nisin production.
- Food wastes containing lactose or glucose were used as feeding substrates.
- The feeding with glucose-containing substrates improved nisin synthesis.
- The feeding with glucose improved the nitrogen utilization for nisin production.
Nisin production by Lactococcus lactis CECT 539 was studied in four realkalized fed-batch cultures in diluted whey with feeding with lactose- and glucose-containing substrates. The first and third cultures were fed with mixtures of whey (W) and a 400Â g/L concentrated glucose (CG), or with a concentrated mussel processing waste (CMPW) and CG, respectively.The second and fourth cultures were respectively performed under the same conditions as in the first and third fermentations. However, these cultures were supplemented with mixtures of W plus a 2% (w/v) yeast extract (WYE2) and CG (second culture), or with CMPW plus a 2% (w/v) yeast extract (CMPWYE2) (fourth culture) after sample extractions at 132 and 168Â h, respectively. From these times, each culture was fed with mixtures of WYE2 and CG, or CMPWYE2 and CG, respectively.The final concentrations of nisin obtained in the third (223.98Â BU/mL) and fourth (350.61Â BU/mL) cultures, fed with glucose-containing substrates (CG and CMPW), were considerably higher than those obtained in the first (108.00Â BU/mL) and second (158.53Â BU/mL) cultures fed with the mixture of lactose- and glucose-containing substrates (W and CG).
Journal: Biochemical Engineering Journal - Volume 123, 15 July 2017, Pages 13-23