کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4752119 | 1415990 | 2017 | 9 صفحه PDF | دانلود رایگان |
- Interaction mechanism of saponins and pancreatic lipase was investigated in vitro.
- The effects of saponins on the reaction kinetics of pancreatic lipase were examined.
- The effects of saponins on the thermodynamics of pancreatic lipase were elucidated.
- The effects of saponins on the conformation of pancreatic lipase were evaluated.
- Morphology and solubility of PL in the presence of saponins was studied.
The molecular interactions between pancreatic lipase (PL) and five triterpenoid saponins compounds, including tea saponin, oleanolic acid, betulin, ginsenoside Ro, and ginsenoside Rd, were investigated in vitro through comprehensive evaluation of PL activity, conformation, reaction kinetics, thermodynamics, aggregation morphology and solubility. All tested saponins inhibited PL activity, and their inhibitory ratios showed structure- and concentration-dependence. Tea saponin possessed competitive inhibition against PL activity, and affected PL secondary structure, while other four saponins non-competitively inhibited PL activity and affected PL tertiary structure. However, all tested saponins could statically quench PL fluorescence. Soluble PL-saponins complexes were characterized by AMF, DLS and Bio-Rad method, and PL showed self-assembly phenomenon in the presence of saponins, which possessed a close contribution to the inhibitory ratios. DSC measurement revealed that five tested saponins compounds had variant influence on PL enzyme thermal stability parameters (Tm and ÎH).
Journal: Biochemical Engineering Journal - Volume 125, 15 September 2017, Pages 1-9