کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4752211 | 1361276 | 2017 | 6 صفحه PDF | دانلود رایگان |

- Succinate-producing E. coli was used to fix CO2 from ethylene oxide off-gas.
- Acetaldehyde was the main factor inhibiting cell metabolic capability.
- irrE gene was introduced to improve cell resistance to acetaldehyde.
- A novel approach to fix CO2 from industrial exhaust gases was established.
Industrial exhaust gases contributed heavily to anthropogenic CO2 emissions. In this study, a novel approach of capturing CO2 from ethylene oxide off-gas and converting it into a platform C4 chemical was established by using succinate-producing E. coli. Fermentation results showed that the acetaldehyde in the off-gas was the main factor inhibiting cell metabolic capability, thus IrrE which is a global regulator from Deinococcus radiodurans was used to improve cell resistance to acetaldehyde. A final succinic acid titer of 68.12 g Lâ1 with a CO2 fixation rate of 4.7 mmol Lâ1 hâ1 was achieved. Notably, this coupled process not only realized the CO2 fixation from ethylene oxide off-gas but also provided a potential cost-effective gas source which holds potential application for bio-based succinate production.
Journal: Biochemical Engineering Journal - Volume 117, Part A, 15 January 2017, Pages 1-6