کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4752228 | 1415999 | 2016 | 5 صفحه PDF | دانلود رایگان |
- We model the relationship between the specific growth rate and osmotic pressure.
- A lactic acid removal system to realize high density cultivation is designed.
- The anion-exchange resins used must be converted to OHâ form.
- The culture system is effective in increasing the biomass of lactic acid bacteria.
A fed-batch culture of Lactobacillus plantarum was coupled with a lactic acid removal system to achieve high-density cultivation. Different types of anion-exchange resins were evaluated to establish the lactic acid removal system. This system comprised a cell bioreactor fitted with two anion-exchange columns: one for adding anion-exchange resins, converted to the OHâ form, to the bioreactor and the other for filtering the resins after adsorption of acid anions. The pH of the culture broth was controlled at between 6.5 and 7.0 by adding anion-exchange resins to remove lactate. In addition, the growth kinetics in the fed-batch and pH-controlled culture were investigated. The well-known Monod equation was modified to describe the relationship between the specific growth rate and the osmotic pressure. In this cultivation system, a cell concentration of 34.5 g Lâ1 was obtained after 12 h, being about 13.3 and 2.3 times higher respectively than that of a batch culture and a fed-batch and pH-controlled culture without the addition of resins. The proposed culture system was effective in increasing the biomass of lactic acid bacteria.
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Journal: Biochemical Engineering Journal - Volume 115, 15 November 2016, Pages 80-84