کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4752759 | 1416369 | 2017 | 39 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Construction of ferrocene modified conducting polymer based amperometric urea biosensor
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Herein, an electrochemical urea sensing bio-electrode is reported that has been constructed by firstly electropolymerizing 4-(2,5-Di(thiophen-2-yl)-1H-pyrrol-1-yl)aniline monomer (SNS-Aniline) on Pencil Graphite Electrode (PGE), then modifying the polymer coated electrode surface with di-amino-Ferrocene (DAFc) as the mediator, and lastly Urease enzyme through glutaraldehyde crosslinking. The effect of pH, temperature, polymer thickness, and applied potential on the electrode current response was investigated besides performing storage and operational stability experiments with the interference studies. The resulting urea biosensor's amperometric response was linear in the range of 0.1-8.5 mM with the sensitivity of 0.54 μA/mM, detection limit of 12 μM, and short response time of 2 s. The designed bio-electrode was tested with real human blood and urine samples where it showed excellent analytical performance with insignificant interference.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Enzyme and Microbial Technology - Volume 102, July 2017, Pages 53-59
Journal: Enzyme and Microbial Technology - Volume 102, July 2017, Pages 53-59
نویسندگان
Muamer Dervisevic, Esma Dervisevic, Mehmet Senel, Emre Cevik, Huseyin Bekir Yildiz, Pınar Camurlu,