کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4753380 | 1416553 | 2017 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Enzymatic synthesis of 2â²-O-methylribonucleosides with a nucleoside hydrolase family enzyme from Lactobacillus buchneri LBK78
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موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
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چکیده انگلیسی
2â²-O-Methylribonucleosides (2â²-OMe-NRs) are promising raw materials for the production of nucleic acid drugs. We previously reported that LbNH, a nucleoside hydrolase from Lactobacillus buchneri LBK78 (NITE P-01581), was the first enzyme found to act on 2â²-OMe-NRs. In the present study, we determined that LbNH also has the transribosylation activity between 2â²-OMe-NRs and nucleobases, in addition to the hydrolyzing activity towards 2â²-OMe-NRs. When 2â²-O-methyluridine (2â²-OMe-UR) and adenine were reacted with LbNH, 2â²-O-methyladenosine (2â²-OMe-AR) was produced. LbNH preferred purine nucleobases as its acceptor substrates for the transribosylation with 2â²-OMe-UR as a donor substrate. Kinetic analysis of LbNH revealed that adenine behaved as a mixed inhibitor of the hydrolysis of 2â²-OMe-UR. Under the optimal reaction conditions, the maximum molar yield of enzymatic 2â²-OMe-AR produced reached 0.97% towards 2â²-OMe-UR, corresponding to 0.16Â g/L.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Bioscience and Bioengineering - Volume 123, Issue 6, June 2017, Pages 659-664
Journal: Journal of Bioscience and Bioengineering - Volume 123, Issue 6, June 2017, Pages 659-664
نویسندگان
Yuuki Mitsukawa, Makoto Hibi, Narihiro Matsutani, Nobuyuki Horinouchi, Satomi Takahashi, Jun Ogawa,