کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4759383 | 1421359 | 2017 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Near-infrared reflectance spectroscopy allows rapid and simultaneous evaluation of chloroplast pigments and antioxidants, carbon isotope discrimination and nitrogen content in Populus spp. leaves
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
بوم شناسی، تکامل، رفتار و سامانه شناسی
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چکیده انگلیسی
Poplar species are economically important sources of timber and bioenergy and they also have a valuable application in phytoremediation and in forest restoration programmes. The characterization of their responses in the face of environmental constraints is essential to ascertain their adaptation capacity. Quantification of chloroplast pigments and antioxidant compounds, nutrients and carbon isotope discrimination (Î13C) of a large set of samples in ecophysiological studies is important for determining the plant's physiological responses. Î13C and nitrogen are parameters of special interest for providing information on photosynthesis, water relations and nutritional characteristics in forest stands. Photoprotective and antioxidant compounds participate in the physiological defence of plants subjected to stressful environmental conditions. The measurement of these leaf components by traditional techniques is laborious and expensive. To evaluate them, a calibration model was obtained using Near-Infrared Reflectance Spectroscopy (NIRS) and chemical analyses of Populus spp. leaves sampled from different clones in different years under different physiological conditions. Predictive calibration equations for the concentration of chloroplast pigments (Chlorophylls a and b, lutein, neoxanthin, β-carotene (β-Car), VAZ (violaxanthin + antheraxanthin + zeaxanthin), epoxidation index (EI = (0.5·antheraxanthin + violaxanthin)/VAZ)), antioxidants (total phenolics (TPhe), ascorbate and α-tocopherol), Î13C and N content were established using a partial least squares regression algorithm. Results showed a remarkable accuracy of the calibration equations for quantifying several antioxidative and photoprotective defence compounds such as neoxanthin (R2 = 0.844), β-Car (R2 = 0.827), VAZ (R2 = 0.846), EI (R2 = 0.831) and excellent accuracy for TPhe (R2 = 0.957), N (R2 = 0.963) and Î13C (R2 = 0.922). As shown, results can be obtained on multiple physiological attributes with a single measurement, saving time and money and demonstrating the suitability of NIRS for ecophysiological purposes and bioremediation programs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Forest Ecology and Management - Volume 399, 1 September 2017, Pages 227-234
Journal: Forest Ecology and Management - Volume 399, 1 September 2017, Pages 227-234
نویسندگان
Jordi Fernà ndez-MartÃnez, Richard Joffre, Massimo Zacchini, Beatriz Fernández-MarÃn, José Ignacio GarcÃa-Plazaola, Isabel Fleck,