Immobilization of Penicillium lilacinum dextranase to produce isomaltooligosaccharides from dextran

Penicillium lilacinum dextranase was immobilized covalently onto Eupergit C and was used for the production of isomaltooligosaccharides. Immobilization resulted in 90% relative activity. The immobilized enzyme showed no decrease in activity for 20 batch reactions. Optimum conditions were not affected by immobilization, and optimum pH and temperature for free and immobilized enzyme were 4.5–5.5 and 30–35 °C, respectively. Immobilized enzyme was more stable at low and high pH and high temperatures. The kinetic parameters for the free and immobilized enzyme were also determined. The immobilized enzyme could be used for the production of isomaltooligosaccharides, since the immobilization efficiency is high (90%) and immobilized enzyme retains its activity for 20 days without any decrease.