کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4853 250 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Partitioning of model proteins in aqueous two-phase systems containing polyethylene glycol and ammonium carbamate
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Partitioning of model proteins in aqueous two-phase systems containing polyethylene glycol and ammonium carbamate
چکیده انگلیسی

The phase equilibrium and the partitioning of bovine serum albumin, trypsin and lysozyme in aqueous two-phase systems containing ammonium carbamate and polyethylene glycol 1500, 4000 and 6000 are presented in this communication. Partition coefficients, defined as the ratio of protein concentration in the polymer phase to that in the salt phase, vary in the range of 0.1–0.8 for bovine serum albumin, 1.0–2.4 for trypsin and from 2.3 to 9.0 for lysozyme, depending on the polymer chain size and on the tie-line length. The analysis of the separation factor, defined as the ratio of partition coefficients of two proteins, shows that high degrees of separation can be achieved. These results indicate that aqueous two-phase systems containing ammonium carbamate and polyethylene glycol can be an alternative to be considered for protein downstream processing.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Engineering Journal - Volume 34, Issue 1, April 2007, Pages 92–97
نویسندگان
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