کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4908053 1426591 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Multiwalled carbonnanotubes enhance the response and sensitivity of the ammonium biosensor based on alanine dehydrogenase
ترجمه فارسی عنوان
کربناته های چند ضلعی تقویت پاسخ و حساسیت بیوسنسور آمونیم بر اساس آلانین دهیدروژناز
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
چکیده انگلیسی


- Carbonnanotubes improved the ammonium biosensor response time from 120 to 30 s.
- Carbonnanotubes improved the linearity range of biosensor (0.05-500 mM NH4+).
- Carbonnanotubes improved detection limit of sensor from 0.1 to 0.001 mM NH4+.
- Multiwalled carbonnanotubes increased the response signal by around 2.4 times.
- Biosensor was validated against Nessler's method by using real and spiked samples.

An amperometric ammonium biosensor based on alanine dehydrogenase of Bacillus subtilis and functionalized multiwalled carbonnanotubes was developed by using screen printed electrodes. The output current of the biosensor was increased around 2.4 times at 100 mM NH4+ after the addition of functionalized multiwalled carbonnanotubes at the concentration of 0.1 μg/ml along with the enzyme. The biosensor showed much broader linearity range (0.05-500 mM NH4+), better detection limit (0.001 mM NH4+) and response time (30 s) due to the incorporation of the functionalized multiwalled carbonnanotubes. The cyclic voltammogram of enzyme-carbonnanotubes/carbon working electrode showed the output current at the oxidation peak, which was > 2 times higher compared to that of enzyme/carbon working electrode. This suggests the promotion of electron transfer from the enzyme to the working electrode by carbonnanotubes, which may act as electron transducers and rapidly transfer the electrons from the redox centre of the enzyme to the electrode, thereby improving the signal, response time and sensitivity. The sensor retained 89.93% of its initial output current after 119 days. The concentrations of NH4+ detected by the biosensor were in close agreement with that of standard Nessler's reagent method suggesting the suitability of the present biosensor for the testing of water samples.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Electroanalytical Chemistry - Volume 784, 1 January 2017, Pages 102-108
نویسندگان
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