کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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4908173 | 1426594 | 2016 | 5 صفحه PDF | دانلود رایگان |
Here, an ultrasensitive electrochemiluminescence (ECL) immunosensor for the detection of cardiac troponin I (cTnI) was developed by using zeolitic imidazolate frameworks encapsulating spherical graphite crystals (CQDs@ZIF-8) as luminophore. Firstly, luminescent spherical graphite crystals (CQDs) were encapsulated in the zeolitic imidazolate framework (ZIF-8) nanocrystals. To improve the poor conductivity of CQDs@ZIF-8, Au nanoparticles were synthesized on the surface of CQDs@ZIF-8 to form AuNPs/CQDs@ZIF-8. Furthermore, L-cysteine (L-cys) as the coreactant of CQDs was immobilized on the AuNPs to enhance the ECL signal of CQDs. Then, the obtained L-cys/AuNPs/CQDs@ZIF-8 was introduced to construct an ultrasensitive ECL immunosensor for the detection of cTnI. As a result, the proposed immunosensor displayed excellent analytical performances for the detection of cTnI with a linear range from 1 fg μL â 1 to 10 pg μLâ 1 and a detection limit of 0.33 fg μLâ 1.
Journal: Journal of Electroanalytical Chemistry - Volume 781, 15 November 2016, Pages 284-288