Analysis of the transient response of a CSTR containing immobilized enzyme particles: Part II. Minimum existence criterion and determination of substrate effective diffusivity and main reaction rate constant

A minimum existence criterion in the transient response of the bulk substrate concentration in a CSTR containing immobilized enzyme (IMEs) in porous solid supports has been obtained from simulation results using several kinetic expressions for the main reaction and the enzyme deactivation reaction. A simple method for the determination of the substrate effective diffusivity and the reaction rate constant is also presented, and applied to the decomposition of hydrogen peroxide, that reacts in a CSTR that contains silica–alumina porous catalyst particles, in which horseradish peroxidase enzyme had been previously immobilized.