Immobilization of Aspergillus niger xylanase A on Fe3O4-coated chitosan magnetic nanoparticles for xylooligosaccharide preparation

• Fe3O4-coated chitosan was prepared by the LBL self-assembly approach.
• XylA was immobilized on Fe3O4-coated chitosan via covalent bond.
• Immobilized XylA can be recycled easily and exhibited improved stability.
• Immobilized XylA released high concentration of XOs from birchwood xylan.

Aspergillus niger xylanase A (XylA) was immobilized onto Fe3O4-coated chitosan magnetic nanoparticles prepared by the layer-by-layer self-assembly approach. The Fe3O4-coated chitosan magnetic nanoparticles showed a high binding capacity of 162.2 mg ∙ g− 1-particles and a recovery activity of 56.5% for XylA. The immobilized XylA showed improved thermostability and storage stability compared with free XylA. The immobilized XylA retained 87.5% activity after seven successive reactions by magnetic separation. Xylotriose and xylohexaose were the main products released from birchwood xylan and wheat bran insoluble xylan by immobilized XylA, respectively.

Figure optionsDownload as PowerPoint slide