کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4980905 | 1367843 | 2016 | 33 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Invertase production and molasses decolourization by cold-adapted filamentous fungus Cladosporium herbarum ER-25 in non-sterile molasses medium
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بهداشت و امنیت شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
This study was undertaken to remove the coloring compounds of molasses as well as produce extracellular (exo) invertase in sterile and non-sterile molasses medium by using cold-adapted filamentous fungus Cladosporium herbarum ER-25. It was determined that a combination of low culture pH (5.5), temperature (20 °C) and high molasses concentration (6%) could completely prevent undesired bacterial contamination during the cultivation of C. herbarum ER-25. Under the optimized non-sterile culture conditions, the maximum invertase activity (36.1 U/mL) was attained after 72 h. On the other hand, the fungus could remove toxical dark brown pigments (melanoidins) in non-sterilized molasses medium through biodegradation and bioadsorption mechanisms. A color removal rate of 64.8% in non-sterile medium could be achieved at the end of 144-h cultivation period. It was found that laccase and manganese peroxidase were responsible for biodegradation. No ligninase activity was detected for the fungus during the cultivation. Maximum laccase (4.6 U/mL) and manganese peroxidase (3.5 U/mL) activities could be reached after 120 h. Higher invertase activity and color removal rate were achieved in non-sterilized medium compared to sterilized one. This is the first report on invertase production from cold-adapted microorganisms under non-sterile culture conditions. As an additional contribution, use of cold-adapted fungi for molasses decolourization was investigated for the first time in the present study.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Safety and Environmental Protection - Volume 103, Part A, September 2016, Pages 136-143
Journal: Process Safety and Environmental Protection - Volume 103, Part A, September 2016, Pages 136-143
نویسندگان
Mesut Taskin, Serkan Ortucu, Yagmur Unver, Ozden Canli Tasar, Mustafa Ozdemir, Haluk Caglar Kaymak,