کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4997315 1459909 2017 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The impact of succinate trace on pWW0 and ortho-cleavage pathway transcription in Pseudomonas putida mt-2 during toluene biodegradation
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
The impact of succinate trace on pWW0 and ortho-cleavage pathway transcription in Pseudomonas putida mt-2 during toluene biodegradation
چکیده انگلیسی


- Succinate repressed toluene catabolism in Pseudomonas putida M9 grown batch cultures.
- Even succinate trace inhibited transcription of the TOL and ortho-cleavage pathway.
- Toluene (pollutant) removal efficiency was notably enhanced.
- Succinate is a dominant repressing substrate for Pseudomonas putida.
- Carbon catabolite repression mechanisms lead to optimal bioremediation strategies.

Toluene is a pollutant catabolised through the interconnected pWW0 (TOL) and ortho-cleavage pathways of Pseudomonas putida mt-2, while upon succinate and toluene mixtures introduction in batch cultures grown on M9 medium, succinate was previously reported as non-repressing. The effect of a 40 times lower succinate concentration, as compared to literature values, was explored through systematic real-time qPCR monitoring of transcriptional kinetics of the key TOL Pu, Pm and ortho-cleavage PbenR, PbenA promoters in mixed-substrate experiments. Even succinate trace inhibited transcription leading to bi-modal promoters expression. Potential carbon catabolite repression mechanisms and novel expression patterns of promoters were unfolded. Lag phase was shortened and biomass growth levels increased compared to sole toluene biodegradation suggesting enhanced pollutant removal efficiency. The study stressed the noticeable effect of a preferred compound's left-over on the main route of a bioprocess, revealing the beneficiary supply of low preferred substrates concentrations to design optimal bioremediation strategies.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 234, June 2017, Pages 397-405
نویسندگان
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