کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4997518 | 1459910 | 2017 | 20 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Transcriptome-based analysis on carbon metabolism of Haematococcus pluvialis mutant under 15% CO2
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
To elucidate the mechanism underlying the enhanced growth rate in the Haematococcus pluvialis mutated with 60Co-γ rays and domesticated with 15% CO2, transcriptome sequencing was conducted to clarify the carbon metabolic pathways of mutant cells. The CO2 fixation rate of mutant cells increased to 2.57 g Lâ1 dâ1 under 15% CO2 due to the enhanced photosynthesis, carbon fixation, glycolysis pathways. The upregulation of PetH, ATPF0A and PetJ related to photosynthetic electron transport, ATP synthase and NADPH generation promoted the photosynthesis. The upregulation of genes related to Calvin cycle and ppdK promoted carbon fixation in both C3 and C4 photosynthetic pathways. The reallocation of carbon was also enhanced under 15% CO2. The 19-, 14- and 3.5-fold upregulation of FBA, TPI and PK genes, respectively, remarkably promoted the glycolysis pathways. This accelerated the conversion of photosynthetic carbon to pyruvate, which was an essential precursor for astaxanthin and lipids biosynthesis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 233, June 2017, Pages 313-321
Journal: Bioresource Technology - Volume 233, June 2017, Pages 313-321
نویسندگان
Ke Li, Jun Cheng, Hongxiang Lu, Weijuan Yang, Junhu Zhou, Kefa Cen,