کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4997949 | 1459918 | 2017 | 10 صفحه PDF | دانلود رایگان |
- Lipase and phytase are produced from Jatropha de-oiled seed cake by C. parapsilosis.
- Seed cake decoction medium is employed for inoculum preparation.
- Toxins and anti-nutrients are reduced significantly from substrate.
- Phorbol esters are eliminated with 3Â days incubation.
Jatropha de-oiled seed cake was explored to utilize as a basic nutrient source for Candida parapsilosis, isolated from poultry garbage and selected based on the production of lipase and phytase enzymes under submerged fermentation. At optimized parameters under solid-state fermentation, lipase and phytase activities were recorded as 1056.66 ± 2.92 and 833 ± 2.5 U/g of substrate (U/g), respectively. Besides enzyme production, complete elimination of phorbol esters and significant phytate reduction from 6.51 ± 0.01 to 0.43 ± 0.01 g/100 g of seed cake were noted after 3 days incubation. Curcin and trypsin inhibition activity were reduced significantly from 26.33 ± 0.43 to 0.56 ± 0.02 mg/100 g and 229.33 ± 2.02 to 11.66 ± 0.28 U/g, respectively after 5 days incubation. Saponins were reduced from 5.56 ± 0.19 to 1.95 ± 0.01 g/100 g of seed cake after 7 days incubation.
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Journal: Bioresource Technology - Volume 225, February 2017, Pages 215-224