کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5030853 1470936 2017 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Lateral flow assay-based bacterial detection using engineered cell wall binding domains of a phage endolysin
ترجمه فارسی عنوان
تشخیص باکتری های مبتنی بر آزمون جریان جانبی با استفاده از دامنه های متصل شده به سلول دیجیتالی مهندسی از یک اندوالیزین فاژ
کلمات کلیدی
باسیلوس سرئوس، باکتریوفاژ، بیوسنسور، دامنه اتصال دیوار سلولی، نوار کاغذی،
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


- The cell wall binding domain (CBD) of endolysin was tested for whole cell detection.
- A lateral flow assay using CBD as a recognition element was developed.
- The assay showed promising results for whole cell detection.

The development of a cost-effective and efficient bacterial detection assay is essential for diagnostic fields, particularly in resource-poor settings. Although antibodies have been widely used for bacterial capture, the production of soluble antibodies is still expensive and time-consuming. Here, we developed a nitrocellulose-based lateral flow assay using cell wall binding domains (CBDs) from phage as a recognition element and colloidal gold nanoparticles as a colorimetric signal for the detection of a model pathogenic bacterium, Bacillus cereus (B. cereus). To improve conjugation efficiency and detection sensitivity, cysteine-glutathione-S-transferase-tagged CBDs and maltose-binding protein-tagged CBDs were produced in Escherichia coli (E. coli) and incorporated in our assays. The sensitivity of the strip to detect B. cereus was 1×104 CFU/mL and the overall assay time was 20 min. The assay showed superior results compared to the antibody-based approach, and did not show any significant cross-reactivity. This proof of concept study indicates that the lateral flow assay using engineered CBDs hold considerable promise as simple, rapid, and cost-effective biosensors for whole cell detection.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 96, 15 October 2017, Pages 173-177
نویسندگان
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