کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5030857 1470936 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Biomolecular stiffness detection based on positive frequency shift of CMOS compatible gigahertz solidly mounted resonators
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Biomolecular stiffness detection based on positive frequency shift of CMOS compatible gigahertz solidly mounted resonators
چکیده انگلیسی
In this work, gigahertz solidly mounted resonators (SMRs) (2.5 GHz) were designed and fabricated to construct a novel particle-resonator system to achieve the biomolecular stiffness sensing in real time. The positive frequency shift of the system was used to estimate the stiffness of biomolecules connecting between the SMR and attached particles. The working principle was revealed by the mathematical analysis of the general block-spring model of the system. Further interpretations about the mechanism of such elastic interaction from the perspective of acoustic resonant modes of SMRs were given by finite element method. Biotin-streptavidin, antibody and antigen binding system were used as model molecular linkers to study the frequency shift varied with different particle diameters and particle densities. Different linker stiffness was realized by adjusting the concentrations of antigens connected with particles which form specific binding with antibodies immobilized on the SMR. The results fairly agree with the simulation results demonstrating the proposed particle-resonator system as an effective method to realize the real-time biomolecular stiffness detection.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 96, 15 October 2017, Pages 206-212
نویسندگان
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