کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5030977 1470935 2017 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
New immobilisation method for oligonucleotides on electrodes enables highly-sensitive, electrochemical label-free gene sensing
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
New immobilisation method for oligonucleotides on electrodes enables highly-sensitive, electrochemical label-free gene sensing
چکیده انگلیسی
We present a versatile and facile procedure for the immobilisation of bioprobe molecules to an electrochemical sensing element. We eliminate lengthy preparation procedures for direct functionalisation of electrode surfaces by pre-attaching probe molecules to carboxylic acid bearing termonomers of pyrrole phenylenes or thiophene phenylenes. We demonstrate that these conjugates can be electrodeposited at low potentials to form nano-scale porous, electroactive conducting polymer films, exposing the bioprobe and retaining activity and specificity for binding, exemplified here with DNA sensors. The electrochemical reaction impedance for Fe(CN)63-/4- on oligonucleotide-modified electrodes showed remarkable (down to aM) detection sensitivity for target DNA sequences present in solution. Cross-sensitivity to non-complementary target sequences is small and multi-target arrays are easily made. There is no need for labelling of either probe or target oligonucleotide.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 97, 15 November 2017, Pages 128-135
نویسندگان
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