کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5031010 1470935 2017 15 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Quantification of antigen by digital domain analysis of integrated nanogap biosensors
ترجمه فارسی عنوان
اندازه گیری آنتی ژن با استفاده از تحلیل دامنه دیجیتال نانوساختارهای یکپارچه
کلمات کلیدی
تجزیه و تحلیل دامنه دیجیتال نانولوله های یکپارچه، نانویوسنسور، ایمونوسنسور،
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Nanogap biosensor shows a distinct conduction change upon sandwich-type immobilization of gold nanoparticle probes onto the gap region in the presence of target biomolecules. Although this large conductance change could be advantageous in distinguishing signal on or off devices, since the extent of conductance change is quite irregular even at the same analyte concentrations, it fails to extract quantitative information from its level of conductance change. In other words, the conductance change of a single device does not reflect the concentration of the target molecule. In this study, we introduce an alternative approach of interpreting the concentration of target molecules using digital domain analysis of integrated nanogap devices, where the fraction of signal-on-devices, or on-device-percentage (ODP), was translated into the concentration of the target molecule. The ODP was found to be closely related to the number density of the immobilized probes and, therefore, to be an excellent measure of the analyte concentration, which was demonstrated in the immuno-selective detection and quantification of influenza A hemagglutinin and prostate specific antigen.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 97, 15 November 2017, Pages 273-277
نویسندگان
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