کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5031108 | 1470938 | 2017 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Label-free electrochemiluminescent biosensor for rapid and sensitive detection of pseudomonas aeruginosa using phage as highly specific recognition agent
ترجمه فارسی عنوان
بیوسنسور الکتروشیمیومومینسانس رایگان برای تشخیص سریع و حساس از سودوموناس آئروژینوزا با استفاده از فاژ به عنوان عامل شناسایی بسیار خاص
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کلمات کلیدی
سودوموناس آئروژینوزا، فاژ، بیوسنسور الکتروشیمیومیلنسنت، گرافن کاربوکسیل، لومینول،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A virulent phage named as PaP1 was isolated from hospital sewage based on a lambda phage isolation protocol. This phage showed a strong and highly specific binding ability to Pseudomonas aeruginosa (P. aeruginosa). Using this isolated phage as a recognition agent, a novel electrochemiluminescent (ECL) biosensor was developed for label-free detection of P. aeruginosa. The biosensor was fabricated through depositing phage-conjugated carboxyl graphene onto the surface of a glass carbon electrode. After specific binding of the host bacteria through the adsorption of P. aeruginosa cell wall by phage tail fibers and baseplate, the ECL signal of luminol suffered a decrease since the formed non-conductive biocomplex obstructed the interfacial electron transfer and blocked the diffusion of the ECL active molecules. The ECL emission declined linearly with P. aeruginosa concentration in the range of 1.4Ã102 â1.4Ã106Â CFUÂ mLâ1, with a very low detection limit of 56Â CFUÂ mLâ1. The whole detection process could be completed within 30Â min as a ready-for-use biosensor was adopted. This biosensor was successfully applied to quantitate P. aeruginosa in milk, glucose injection and human urine with acceptable recovery values ranging from 78.6% to 114.3%.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 94, 15 August 2017, Pages 429-432
Journal: Biosensors and Bioelectronics - Volume 94, 15 August 2017, Pages 429-432
نویسندگان
Huan Yue, Yong He, Enci Fan, Lin Wang, Shuguang Lu, Zhifeng Fu,