کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5031161 | 1470941 | 2017 | 30 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Electrochemical bioassay development for ultrasensitive aptasensing of prostate specific antigen
ترجمه فارسی عنوان
توسعه زیست سنجی الکتروشیمیایی برای تصفیه فوق حساسیتی آنتی ژن خاص پروستات
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
آپتامر، آنتی ژن اختصاصی پروستات، طیف سنجی امپدانس الکتروشیمیایی، تشخیص رایگان برچسب نانولوله های کربنی چند ضلعی،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A densely packed gold nanoparticles on the rGO-MWCNT platform was used as the basis for an ultrasensitive label-free electrochemical aptasensor to detect the biomarker prostate specific antigen (PSA) in serum. The detection was based on that the variation of electron transfer resistance (Rct) and differential pulse voltammetry (DPV) current were relevant to the formation of PSA-aptamer complex at the modified electrode surface. Compared with pure AuNPs, rGO-MWCNT and MWCNT/AuNPs, the rGO-MWCNT/AuNPs nanocomposite modified electrode was the most sensitive aptasensing platform for the determination of PSA. Two calibration curves were prepared from the data obtained from the DPV and electrochemical impedance spectroscopy (EIS) by plotting the peak current and Rct against PSA concentration, respectively. The proposed aptasensor had an extremely low LOD of 1.0 pg mLâ1 PSA within the detection range of 0.005-20 ng mLâ1 and 0.005-100 ng mLâ1 for DPV and EIS calibration curves, respectively. This sensor exhibited outstanding anti-interference ability towards co-existing molecules with good stability, sensitivity, and reproducibility. Clinical application was performed with analysis of the PSA levels in serum samples obtained from patients with prostate cancer using both the aptasensor and Immunoradiometric assay. The results revealed the proposed system to be a promising candidate for clinical analysis of PSA.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 91, 15 May 2017, Pages 284-292
Journal: Biosensors and Bioelectronics - Volume 91, 15 May 2017, Pages 284-292
نویسندگان
Esmaeil Heydari-Bafrooei, Nazgol Sadat Shamszadeh,