کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5031190 | 1470941 | 2017 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
An ultrasensitive aptasensor for Ochratoxin A using hexagonal core/shell upconversion nanoparticles as luminophores
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We developed an ultrasensitive luminescence resonance energy transfer (LRET) aptasensor for Ochratoxin A (OTA) detection, using core/shell upconversion nanoparticles (CS-UCNPs) as luminophores. The OTA aptamer was tagged to CS-UCNPs as energy donor and graphene oxide (GO) acted as energy acceptor. The Ï-Ï stacking interaction between the aptamer and GO brought CS-UCNPs and GO in close proximity hence initiated the LRET process resulting in quenching of CS-UCNPs luminescence. A linear calibration was obtained between the luminescence intensity and the logarithm of OTA concentration in the range from 0.001 ng mLâ1 to 250 ng mLâ1, with a detection limit of 0.001 ng mLâ1. The aptasensor showed good specificity towards OTA in beer samples. The ultrahigh sensitivity and pronounced robustness in beer sample matrix suggested promising prospect of the aptasensor inpractical applications.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 91, 15 May 2017, Pages 538-544
Journal: Biosensors and Bioelectronics - Volume 91, 15 May 2017, Pages 538-544
نویسندگان
Shaoliang Dai, Shijia Wu, Nuo Duan, Jian Chen, Zhigao Zheng, Zhouping Wang,