کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5031216 | 1470941 | 2017 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Lab on a fabric: Mass producible and low-cost fabric filters for the high-throughput viable isolation of circulating tumor cells
ترجمه فارسی عنوان
آزمایشگاهی روی یک پارچه: فیلترهای پارچه ای توده ای تولید شده و کم هزینه برای جداسازی قابل انعطاف باالی پروتئین های سلول های توموری
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کلمات کلیدی
سلول های تومور گردش خون، فیلتر پارچه، انزوا حیاتی، مطالعه بالینی سرطان، ابزار تشخیص سرطان ارزان قیمت، وسیع پزشکی تولیدی،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Circulating tumor cells (CTCs) play an important role in estimating the presence and the metastatic relapse of tumor. Despite of their importance, isolation of viable CTCs is still struggling, since chemical or mechanical damages are unavoidable when separating less than 1000 of CTCs out of billions of other blood components. Furthermore, the current CTC isolation devices show low productivity, since they are produced after a series of complicated fabrication processes. Here, we present a low-cost and mass-producible fabric filters for the viable CTC isolation and the further molecular assay for profiling cancer-associated markers. The fabric filter, produced by polyester monofilament yarns, can be massively produced at extremely low-cost, by showing productivity of ~22Â filters/s at ~59Â filters/USD. By utilizing size-based sorting method, the fabric filter is capable to isolate both epithelial and mesenchymal CTCs, while slots with curved walls are beneficial for preventing the cell rupture by reducing 21.6% of mechanical stress compared to the conventional straight-walled slots. We applied our filter to 11 human blood samples and found that the number of CTCs was closely related to the expression level of Ki-67, which is highly overexpressed in proliferative tumors. The fabric filter might be an appropriate caner-screening tool in developing countries, where people suffer from insufficient healthcare services.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 91, 15 May 2017, Pages 747-755
Journal: Biosensors and Bioelectronics - Volume 91, 15 May 2017, Pages 747-755
نویسندگان
Jiyoon Bu, Yoon-Tae Kang, Yong-Seok Lee, Jeongsuk Kim, Young-Ho Cho, Byung-In Moon,