کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5031236 1470942 2017 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Molecular visualizing and quantifying immune-associated peroxynitrite fluxes in phagocytes and mouse inflammation model
ترجمه فارسی عنوان
تجسم مولکولی و کوانتوم های فلوئور پراکسینیتریت مرتبط با ایمنی در مدل فاگوسیت ها و التهاب موش
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


- F482 was applied in ratiometrically detecting ONOO-.
- Carboxylic BODIPY formation by ONOO--induced diene oxidation was confirmed.
- F482 enables us to quantify ONOO- in phagosomes by high-throughput flow cytometry.
- Visualizing and quantifying ONOO- fluxes in mouse inflammation model was realized.

Reactions of peroxynitrite (ONOO−) with biomolecules can lead to cytotoxic and cytoprotective events. Due to the difficulty of directly and unambiguously measuring its levels, most of the beneficial effects associated with ONOO− in vivo remain controversial or poorly characterized. Recently, optical imaging has served as a powerful noninvasive approach to studying ONOO− in living systems. However, ratiometric probes for ONOO− are currently lacking. Herein, we report the design, synthesis, and biological evaluation of F482, a novel fluorescence indicator that relies on ONOO−-induced diene oxidation. The remarkable sensitivity, selectivity, and photostability of F482 enabled us to visualize basal ONOO− in immune-stimulated phagocyte cells and quantify its generation in phagosomes by high-throughput flow cytometry analysis. With the aid of in vivo ONOO− imaging in a mouse inflammation model assisted by F482, we envision that F482 will find widespread applications in the study of the ONOO− biology associated with physiological and pathological processes in vitro and in vivo.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 90, 15 April 2017, Pages 75-82
نویسندگان
, , , , , , , ,