کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5031236 | 1470942 | 2017 | 8 صفحه PDF | دانلود رایگان |
- F482 was applied in ratiometrically detecting ONOO-.
- Carboxylic BODIPY formation by ONOO--induced diene oxidation was confirmed.
- F482 enables us to quantify ONOO- in phagosomes by high-throughput flow cytometry.
- Visualizing and quantifying ONOO- fluxes in mouse inflammation model was realized.
Reactions of peroxynitrite (ONOOâ) with biomolecules can lead to cytotoxic and cytoprotective events. Due to the difficulty of directly and unambiguously measuring its levels, most of the beneficial effects associated with ONOOâ in vivo remain controversial or poorly characterized. Recently, optical imaging has served as a powerful noninvasive approach to studying ONOOâ in living systems. However, ratiometric probes for ONOOâ are currently lacking. Herein, we report the design, synthesis, and biological evaluation of F482, a novel fluorescence indicator that relies on ONOOâ-induced diene oxidation. The remarkable sensitivity, selectivity, and photostability of F482 enabled us to visualize basal ONOOâ in immune-stimulated phagocyte cells and quantify its generation in phagosomes by high-throughput flow cytometry analysis. With the aid of in vivo ONOOâ imaging in a mouse inflammation model assisted by F482, we envision that F482 will find widespread applications in the study of the ONOOâ biology associated with physiological and pathological processes in vitro and in vivo.
Journal: Biosensors and Bioelectronics - Volume 90, 15 April 2017, Pages 75-82