کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5031630 | 1470940 | 2017 | 15 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Detection of the tau protein in human serum by a sensitive four-electrode electrochemical biosensor
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Detection of the tau protein in human serum by a sensitive four-electrode electrochemical biosensor Detection of the tau protein in human serum by a sensitive four-electrode electrochemical biosensor](/preview/png/5031630.png)
چکیده انگلیسی
This study presents a novel approach based on a four-electrode electrochemical biosensor for the detection of tau protein - one of the possible markers for the prediction of Alzheimer's disease (AD). The biosensor is based on the formation of stable antibody-antigen complexes on gold microband electrodes covered with a layer of a self-assembled monolayer and protein G. Antibodies were immobilized on the gold electrode surface in an optimal orientation by protein G interaction. Electrochemical impedance spectroscopy was used to analyze impedance change, which revealed a linear response with increasing tau concentrations. The assay is fast (<1Â h for incubation and measurement) and very sensitive. The limit of quantification for the full-length 2N4R tau protein is 0.03Â pM, a value unaltered when the assay was processed in bovine serum albumin or human serum. This technology could be adapted for the detection of other biomarkers to provide a multiple assay to identify AD progression in a point of care setting.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 92, 15 June 2017, Pages 482-488
Journal: Biosensors and Bioelectronics - Volume 92, 15 June 2017, Pages 482-488
نویسندگان
Scarlet Xiaoyan Wang, Desiree Acha, Ajit J. Shah, Frank Hills, Ivan Roitt, Andreas Demosthenous, Richard H. Bayford,