کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5031728 | 1369954 | 2017 | 27 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
An electrochemical biosensor to simultaneously detect VEGF and PSA for early prostate cancer diagnosis based on graphene oxide/ssDNA/PLLA nanoparticles
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کلمات کلیدی
Single strand DNA - DNA تک رشته ایProstate-specific antigen (PSA) - آنتی ژن اختصاصی پروستات (PSA)Graphene oxide - اکسید گرافنElectrochemical biosensor - بیوسنسور الکتروشیمیایی یا زیست حسگرهای الکتروشیمیایی Prostate cancer (PCa) - سرطان پروستات (PCa)Vascular endothelial growth factor (VEGF) - فاکتور رشد اندوتلیال عروقی (VEGF)
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: An electrochemical biosensor to simultaneously detect VEGF and PSA for early prostate cancer diagnosis based on graphene oxide/ssDNA/PLLA nanoparticles An electrochemical biosensor to simultaneously detect VEGF and PSA for early prostate cancer diagnosis based on graphene oxide/ssDNA/PLLA nanoparticles](/preview/png/5031728.png)
چکیده انگلیسی
Early diagnosis of prostate cancer (PCa) is critical for the prevention of metastasis and for early treatment; therefore, a simple and accurate device must be developed for this purpose. In this study, we reported a novel fabrication method for producing a dual-modality biosensor that can simultaneously detect vascular endothelial growth factor (VEGF) and prostate-specific antigen (PSA) in human serum for early diagnosis of PCa. This biosensor was constructed by coating graphene oxide/ssDNA (GO-ssDNA) on an Au-electrode for VEGF detection, and incorporated with poly-L-lactide nanoparticles (PLLA NPs) for signal amplification and PSA detection. The results showed that this biosensor has wide liner detection ranges (0.05-100Â ng/mL for VEGF and 1-100Â ng/mL for PSA), as well as high levels of sensitivity and selectivity (i.e., resisting interference from external factors, such as glucose, ascorbic acid human serum protein, immunoglobulin G, and immunoglobulin M), and demonstrated a high correlation with an enzyme-linked immunosorbent assay for sample detection in patients. Therefore, this biosensor could be utilized for early clinical diagnosis of PCa in the future.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 89, Part 1, 15 March 2017, Pages 598-605
Journal: Biosensors and Bioelectronics - Volume 89, Part 1, 15 March 2017, Pages 598-605
نویسندگان
Lung-Hsuan Pan, Shin-Hung Kuo, Tzu-Yang Lin, Chih-Wen Lin, Po-Yu Fang, Hung-Wei Yang,