Lab on paper chip integrated with Si@GNRs for electroanalysis of diazepam

- EμPAD offers many advantageous features such as facile approach, economic and have potential for commercialization.
- Extensive development can be made for industrial translation of this fabricated device.
- Si@GNR modified EμPAD showed wide linear range of 3.5 nM to 3.5 mM.
- The detection limit was as low as 1.5 x 10−9 M for diazepam detection.
- The developed sensor was tested in real time samples like injection, tablets and found good correlation.

We describe herein the fabrication of an electrochemical microfluidic paper based device (EμPAD) for the detection of diazepam, a sedative, anxiety-relieving and muscle-relaxing drug. To achieve it, silica coated gold nanorods (Si@GNRs) were synthesized and drop casted on an electrochemical microfluidic paper based device (EμPAD) for the detection of diazepam. The synthesized composites were characterized by recording its images in scanning electron microscope (SEM) and transmission electron microscope (TEM). The experimental results confirmed that Si@GNRs had good electrocatalytic activity towards diazepam. The modified paper based electrode showed a stable electrochemical response for diazepam in the concentration range of 3.5 nM to 3.5 mM. EμPAD offers many advantageous features such as facile approach, economical and have potential for commercialization. Si@GNRs modified EμPAD was also employed for determination of diazepam in spiked human urine samples. Reported facile lab paper approach integrated with Si@GNRs could be well applied for the determination of serum metabolites.

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