کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5130726 1490847 2017 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Quantum-dot nanoprobes and AOTF based cross talk eliminated six color imaging of biomolecules in cellular system
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Quantum-dot nanoprobes and AOTF based cross talk eliminated six color imaging of biomolecules in cellular system
چکیده انگلیسی


- Quantum dot (Q-dot) and acousto-optic tunable filters (AOTF) based six-colour imaging.
- Expression of PPAR-γ in adipocyte regulates insulin resistance in hepatic (HepG2) cells.
- Aspirin improved insulin sensitivity in adipocytes and HepG2 co-cell culture.

Primary cell cultures mimic the physiology and genetic makeup of in-vivo tissue of origin, nonetheless, a complication in the derivation and propagation of primary cell culture limits its use in biological research. However, in-vitro models using primary cells might be a complement model to mimic in vivo response. But, conventional techniques such as western blot and PCR employed to study the expression and activation of proteins requires a large number of cells, hence repeated establishment and maintenance of primary culture are unavoidable. Quantum dot (Q-dot) and acousto-optic tunable filters (AOTF) based multiplex imaging system is a viable alternative choice to evaluate multiple signaling molecules by using a small number of cells. Q-dots have broad excitation and narrow emission spectra, which allows to simultaneously excite multiple Q-dots by using single excitation wavelength. The use of AOTF in the fluorescence detection system enables to scan the fluorescence emission intensity of a Q-dot at their central wavelength, this phenomenon effectively avoids spectral overlap among the neighboring Q-dots. When Q-dots are conjugated with antibodies it acts as effective sensing probes. To validate this, the expression pattern of p-JNK-1, p-GSK3β, p-IRS1ser, p-IRS1tyr, p-FOXO1, and PPAR-γ, involved in the insulin resistance were concurrently monitored in adipocyte and HepG2 co-cell culture model. The observed results clearly indicate that PPAR-γ is the critical component in the development of insulin resistance. Moreover, the results proved that developed Q-dot based AOTF imaging methodology is a sensible choice to concurrently monitor multiple signaling molecules with limited cell population.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 985, 8 September 2017, Pages 166-174
نویسندگان
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