کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5130826 1490848 2017 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Saturated fatty acid determination method using paired ion electrospray ionization mass spectrometry coupled with capillary electrophoresis
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Saturated fatty acid determination method using paired ion electrospray ionization mass spectrometry coupled with capillary electrophoresis
چکیده انگلیسی


- A new CE- PIESI MS method was developed to determine a wide range of fatty acids.
- It provides excellent sensitivity with good peak resolution for short-chain fatty acids.
- The method was validated and provided good linearity and repeatability.
- The amounts of fatty acids in cheese and coffee extracts were determined.

A sensitive and selective capillary electrophoresis-mass spectrometry (CE-MS) method for determination of saturated fatty acids (FAs) was developed by using dicationic ion-pairing reagents forming singly charged complexes with anionic FAs. For negative ESI detection, 21 anionic FAs at pH 10 were separated using ammonium formate buffer containing 40% acetonitrile modifier in normal polarity mode in CE by optimizing various parameters. This method showed good separation efficiency, but the sensitivity of the method to short-chain fatty acids was quite low, causing acetic and propionic acids to be undetectable even at 100 mgL−1 in negative ESI-MS detection. Out of the four dicationic ion-pairing reagents tested, N,N'-dibutyl 1,1'-pentylenedipyrrolidium infused through a sheath-liquid ion source during CE separation was the best reagent regarding improved sensitivity and favorably complexed with anionic FAs for detection in positive ion ESI-MS. The monovalent complex showed improved ionization efficiency, providing the limits of detection (LODs) for 15 FAs ranging from 0.13 to 2.88 μg/mL and good linearity (R2 > 0.99) up to 150 μg/mL. Compared to the negative detection results, the effect was remarkable for the detection of short- and medium-chain fatty acids. The optimized CE-paired ion electrospray (PIESI)-MS method was utilized for the determination of FAs in cheese and coffee with simple pretreatment. This method may be extended for sensitive analysis of unsaturated fatty acids.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 984, 1 September 2017, Pages 223-231
نویسندگان
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