کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5130925 | 1490869 | 2017 | 7 صفحه PDF | دانلود رایگان |
- A novel dose-dependent multicolor conversion strategy is developed for colorimetric bioassays.
- AuNP-mediated copper deposition is employed for signal amplification.
- Semi-quantitative PSA detection was demonstrated with naked eyes.
- The sensing platform can be easily adapted to detection of other biomolecules.
Colorimetric immunoassays have been attracting more attention for use in practical applications, especially in pointâofâcare diagnostics. In comparison with a single color immunoassay, the doseâdependent multicolor strategy greatly improves the detection resolution and accuracy of visual inspection. In the current study, a concentrationâdependent multicolor conversion strategy was developed based on gold nanoparticle (AuNP)âmediated copper deposition for signal amplification and Prussian blue for color generation. Under optimal conditions, a doseâdependent multicolor from yellow through green to blue were successfully achieved, which was easier to be differentiated from each other by the naked eyes. With rabbit IgG and prostate specific antigen (PSA) as model analytes, semiâquantitative evaluations were demonstrated in lab buffer and serum by direct readout with the naked eyes. Quantitative detections were also accomplished by measurement of absorbance of Prussian blue with a common UV-Vis spectrophotometer. A limit of detection (LOD) down to subâpicogram per milliliter was determined. In addition, this newly developed colorimetric assay method can be easily adapted for the detection of other biomolecules by simply changing the recognition pairs.
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Journal: Analytica Chimica Acta - Volume 963, 22 April 2017, Pages 129-135