Determination of formylated DNA and RNA by chemical labeling combined with mass spectrometry analysis

- Chemical labeling-MS analysis was developed for study of nucleic acid formylation.
- The detection sensitivities of formylated nucleosides increased by 307-884 folds.
- Six formylated nucleosides from both DNA and RNA were detected.
- Three formylated nucleosides were firstly discovered in human cells and tissues.
- Significant increase of two formylated nucleosides in thyroid carcinoma tissues.

Nucleic acids carry diverse chemical modifications that exert critical influences in a variety of cellular processes in living organisms. In addition to methylation, the emerging DNA and RNA formylation has been reported to play functional roles in various physiological processes. However, the amounts of formylated DNA and RNA are extremely low and detection of DNA and RNA formylation is therefore a challenging task. To address this issue, we developed a strategy by chemical labeling combined with in-tube solid-phase microextraction - ultra high performance liquid chromatography - electrospray ionization - tandem mass spectrometry (in-tube SPME-UPLC-ESI-MS/MS) analysis for the sensitive determination of DNA and RNA formylation. Using the developed method, we were able to simultaneously measure six formylated nucleosides, including 5-formyl-2′-deoxycytidine (5-fodC), 5-formylcytidine (5-forC), 5-formyl-2′-deoxyuridine (5-fodU), 5-formyluridine (5-forU), 2′-O-methyl-5-formylcytidine (5-forCm) and 2′-O-methyl-5- formyluridine (5-forUm), from DNA and RNA of cultured human cells and multiple mammalian tissues. The detection limits of these formylated nucleosides improved by 307-884 folds using Girard's P (GirP) labeling coupled with in-tube SPME-UPLC-ESI-MS/MS analysis. It was worth noting that 5-forU, 5-forCm and 5-forUm which have not been detected in human sample before, were discovered in cultured human cells and tissues in the current study. In addition, we observed significant increase of 5-forC and 5-forU in RNA (p = 0.027 for 5-forC; p = 0.028 for 5-forU) and 5-fodU in DNA (p = 0.002) in human thyroid carcinoma tissues compared to normal tissues adjacent to the tumor using synthesized stable isotope GirP (d5-GirP)-assisted quantification. Our results indicated that aberrant DNA and RNA formylation may contribute to the tumor formation and development. In addition, monitoring of DNA and RNA formylation may also serve as indicator for cancer diagnostics. Taken together, the developed chemical labeling combined with in-tube SPME-UPLC-ESI-MS/MS analysis can facilitate the in-depth functional study of DNA and RNA formylation.

197