کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5131054 | 1490862 | 2017 | 9 صفحه PDF | دانلود رایگان |

- Polyethyleneimine/citric acid co-carbonized dots were prepared.
- Zn2+ can enhance fluorescence of C-dots by inhibiting PET pathways.
- Co2+ can quench the enhanced fluorescence by an inner filter effect.
- Bioprobe has been established for intracellular imaging Co.
Whether as an important biological element or as a radioactive source/medicine, the monitoring of trace levels of cobalt ions (Co) has become a non-negligible factor for human health and green environment. Current technologies for the detection of Co are cost-expensive and time-consuming, and require cumbersome sample pretreatment process. Herein a novel sensing platform has been developed for Co detection based on the quenching of the enhanced fluorescence signal of polyamine functionalized C-dots. Amine groups at the surface of the C-dots can capture Zn2+/Cd2+ to form coordination compound, which can inhibit the photoinduced electron transfer pathways of C-dots and then induce the fluorescence enhancement of the C-dots by â¼80% margin. Also, Co interacts with these amine groups to form an absorbent complex, which can strongly quench the enhanced fluorescence of C-dots via an inner filter effect. This C-dots-based probe showed a wide linear response to Co with a concentration ranging from 0.012 to 12 μM, and a detection limit of 8.0 nM and RSD of 5.7% (n = 5). Significantly, the C-Dots exhibit excellent properties, such as negligible cytotoxicity, excellent biocompatibility, low-cost and high photostability, etc., which make C-dots favorable for label-free monitoring of Co and then successfully applied to the confocal imaging of intracellular Co.
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Journal: Analytica Chimica Acta - Volume 970, 1 June 2017, Pages 64-72