کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5131347 | 1490881 | 2017 | 10 صفحه PDF | دانلود رایگان |
- An novel DNA biosensor was constructed based on GONs with deposited AgNPs.
- GONs catalyze the in-situ deposition of AgNPs on the sensing interface.
- Unique Ï-stacking of GONs with probe DNA contributes high selectivity of the biosensor.
- High electroactivity of AgNPs leads to low detection limit (7.6Â fM) for target DNA.
The development of high-performance biosensing platform is heavily dependent on the recognition property of the sensing layer and the output intensity of the signal probe. Herein, we present a simple and highly sensitive biosensing interface for DNA detection on the basis of graphene oxide nanosheets (GONs) directed in-situ deposition of silver nanoparticles (AgNPs). The fabrication process and electrochemical properties of the biosensing interface were probed by electrochemical techniques and scanning electron microscopy. The results indicate that GONs can specifically adsorb at the single-stranded DNA probe surface, and induces the deposition of highly electroactive AgNPs. Upon hybridization with complementary oligonucleotides to generate the duplex DNA on the electrode surface, the GONs with the deposited AgNPs will be liberated from the sensing interface due to the inferior affinity of GONs and duplex DNA, resulting in the reduction of the electrochemical signal. Such a strategy combines the superior recognition of GONs toward single-stranded DNA and double-stranded DNA, and the strong electrochemical response of in-situ deposited AgNPs. Under optimal conditions, the biosensor can detect target DNA over a wide range from 10Â fM to 10Â nM with a detection limit of 7.6Â fM. Also, the developed biosensor shows outstanding discriminating ability toward oligonucleotides with different mismatching degrees.
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Journal: Analytica Chimica Acta - Volume 951, 25 January 2017, Pages 58-67