Performance of hybrid electronic tongue and HPLC coupled with chemometric analysis for the monitoring of yeast biotransformation

- The first application of hybrid electronic tongue for the monitoring of yeast biotransformation.
- Optical density, glucose and 2- phenylethanol concentration determined with high accuracy and precision.
- Analysis of electrochemical and chromatographic fingerprints by Partial Least Squares analysis.
- The first comparison of two non-selective analytical systems in bioprocess monitoring.

Monitoring of process parameters is of great importance in the field of bioprocess control. In this work two analytical systems: hybrid electronic tongue combining potentiometric and voltammetric detection (hET) and HPLC coupled with Partial Least Squares (PLS) analysis (HPLC-PLS) have been evaluated and compared as novel, rapid techniques for biotransformation monitoring. They were applied for the analysis of yeast culture media during batch fermentation carried out for 2-phenylethanol production. Work-flow of numerical analysis of sample fingerprints provided by these both techniques was presented. The ability of hET-PLS and HPLC-PLS to predict main process parameters, such as optical density, time of culture, glucose and 2-phenylethanol concentration was studied. Both non-selective analytical systems revealed to be suitable tools for yeast fermentation monitoring, while slightly better results were obtained by hET-PLS.

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