Application of dietary fiber method AOAC 2011.25 in fruit and comparison with AOAC 991.43 method

- Quantification of dietary fiber in fruit by the AOAC 2011.25 and AOAC 991.43 methods.
- Efficiency of the AOAC 2011.25 method in fruit was analyzed.
- The AOAC 2011.25 method provides higher dietary fiber values for fruits containing fructans.
- Non-digestible oligosaccharides make the application of the AOAC 2011.25 method relevant.

AOAC 2011.25 method enables the quantification of most of the dietary fiber (DF) components according to the definition proposed by Codex Alimentarius. This study aimed to compare the DF content in fruits analyzed by the AOAC 2011.25 and AOAC 991.43 methods. Plums (Prunus salicina), atemoyas (Annona x atemoya), jackfruits (Artocarpus heterophyllus), and mature coconuts (Cocos nucifera) from different Brazilian regions (3 lots/fruit) were analyzed for DF, resistant starch, and fructans contents. The AOAC 2011.25 method was evaluated for precision, accuracy, and linearity in different food matrices and carbohydrate standards. The DF contents of plums, atemoyas, and jackfruits obtained by AOAC 2011.25 was higher than those obtained by AOAC 991.43 due to the presence of fructans. The DF content of mature coconuts obtained by the same methods did not present a significant difference. The AOAC 2011.25 method is recommended for fruits with considerable fructans content because it achieves more accurate values.