Different inhibition mechanisms of gentisic acid and cyaniding-3-O-glucoside on polyphenoloxidase

- Inhibitory effect of C3G on PPO was much stronger than that of GA.
- GA reversibly inhibited PPO in mixed-type while C3G was an irreversible inhibitor.
- C3G had stronger effects on thermal inactivation and conformation of PPO than GA.
- GA bound to PPO by hydrogen bond, π-π stacking and van der Waals forces.
- C3G irreversibly interacted with Cys or Met in PPO by covalent bonds.

Gentisic acid and cyanidin-3-O-glucoside are important bioactive polyphenols which are widely distributed in many fruits and cereals. In this work, kinetic study, spectral analysis and computational simulation were used to compare the inhibitory effects and inhibition mechanisms of gentisic acid and cyanidin-3-O-glucoside on mushroom polyphenoloxidase (PPO). The inhibitory effect of cyanidin-3-O-glucoside on PPO was much stronger than that of gentisic acid. Gentisic acid inhibited PPO in a reversible mixed-type manner while cyanidin-3-O-glucoside was an irreversible inhibitor. Gentisic acid and cyanidin-3-O-glucoside made the thermal inactivation of PPO easier, and induced apparent conformational changes of PPO. Compared with gentisic acid, cyanidin-3-O-glucoside had stronger effects on the thermal inactivation and conformation of PPO. Molecular docking results revealed gentisic acid bound to the active site of PPO by hydrogen bonding, π-π stacking and van der Waals forces. However, cyanidin-3-O-glucoside might irreversibly interact with the Met or Cys in PPO by covalent bonds.