Rapid determination of flavonoids and phenolic acids in grape juices and wines by RP-HPLC/DAD: Method validation and characterization of commercial products of the new Brazilian varieties of grape

- A method for rapid determination of phenolic compounds by RP-HPLC was validated.
- Method used to characterize commercial products from new grape Brazilian varieties.
- The method showed good parameters in a run time of only 25 min.
- Wine and grape juices analyzed showed high antioxidant activity.

A method for rapid determination of phenolic compounds by reversed-phase high-performance liquid chromatography (RP-HPLC), using a new column of faster resolution was validated and used to characterize commercial products produced with new grape Brazilian varieties of Northeast of Brazil. The in vitro antioxidant activity was also measured. The method showed linearity (R > 0.9995), good precision (CV% < 2.78), recovery (91.8-105.1%) and limits of detection (0.04-0.85 mg L−1) and quantification (0.04-1.41 mg L−1) according to other methods previously published with the difference of a run time of only 25 min. The results obtained in the characterization of the samples differed for juices and wines from other world regions, mainly because of the high values of (−)-epigallocatechin and trans-caftaric acid. The products analyzed showed high antioxidant activity, especially the wine samples with values higher than those from wines of different regions of the world.