کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5134970 | 1493407 | 2017 | 7 صفحه PDF | دانلود رایگان |

- A novel on-line mixed-mode gel liquid chromatography Ã reversed phase liquid chromatography method was developed.
- Size exclusion, reversed phase partition and adsorption separation mechanisms were involved in the first dimension.
- A new interface solution was designed for the new 2D-LC system.
- The new 2D-LC system exhibited better orthogonality (72.9%), better separation resolution and peak capacity.
A novel on-line comprehensive two-dimensional liquid chromatography (2D-LC) method by coupling mixed-mode gel liquid chromatography (MMG-LC) with reversed phase liquid chromatography (RPLC) was developed. A mixture of 17 reference compounds was used to study the separation mechanism. A crude water extract of Flos Carthami was applied to evaluate the performance of the novel 2D-LC system. In the first dimension, the extract was eluted with a gradient of water/methanol over a cross-linked dextran gel Sephadex LH-20 column. Meanwhile, the advantages of size exclusion, reversed phase partition and adsorption separation mechanism were exploited before further on-line reversed phase purification on the second dimension. This novel on-line mixed-mode Sephadex LH-20Â ÃÂ RPLC method provided higher peak resolution, sample processing ability (2.5Â mg) and better orthogonality (72.9%) versus RPLCÂ ÃÂ RPLC and hydrophilic interaction liquid chromatography (HILIC)Â ÃÂ RPLC. To the best of our knowledge, this is the first report of a mixed-mode Sephadex LH-20Â ÃÂ RPLC separation method with successful applications in on-line mode, which might be beneficial for harvesting targets from complicated medicinal plants.
Journal: Journal of Chromatography A - Volume 1519, 13 October 2017, Pages 145-151