کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5134972 | 1493407 | 2017 | 6 صفحه PDF | دانلود رایگان |
- AF4 allows the efficient separation of nanoparticle populations of similar sizes.
- Chemical identification of different nano-sized species in complex mixtures achieved.
- The DHLA surface-capping procedure directly produces purified QDs.
Coupling of asymmetric flow field-flow fractionation (AF4) to an on-line elemental detection (inductively coupled plasma-mass spectrometry, ICP-MS) has been recently proposed as a powerful diagnostic tool for characterization of the bioconjugation of CdSe/ZnS core-shell Quantum Dots (QDs) to antibodies. Such approach has been used herein to demonstrate that cap exchange of the native hydrophobic shell of core/shell QDs with the bidentate dihydrolipoic acid ligands directly removes completely the eventual side nanoparticulated populations generated during simple one-pot synthesis, which can ruin the subsequent final bioapplication. The critical assessment of the chemical and physical purity of the surface-modified QDs achieved allows to explain the transmission electron microscopy findings obtained for the different nanoparticle surface modification assayed.
Journal: Journal of Chromatography A - Volume 1519, 13 October 2017, Pages 156-161