کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5134980 1493414 2017 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Molecular insight into protein binding orientations and interaction modes on hydrophobic charge-induction resin
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Molecular insight into protein binding orientations and interaction modes on hydrophobic charge-induction resin
چکیده انگلیسی


- Ligand density impact on HCIC was elucidated with molecular simulation.
- Orientations effects on adsorption were studied.
- Conformation adjustment and multiple-site binding were found at molecular level.
- Desorption behaviors were studied and analyzed at molecular level.

Hydrophobic charge-induction chromatography (HCIC) with 4-mercaptoethyl-pyridine (MEP) as the functional ligand has been developed as a new technology for antibody purification. In the present work, molecular simulation methods were developed to investigate the interactions between the Fc fragment of IgG and a MEP ligand net. The MM/PBSA method was used to evaluate the binding energy for the MEP ligand net at different densities. It was found that ligand density had significant influence on the binding of Fc. Potential binding conformations were further analyzed by molecular dynamics simulation. It was found that the interaction between Fc and MEP ligand net is driven by self-adaptive conformation adjustment and multiple-site binding. Hydrophobic forces dominate the binding interaction, which appeared as the results of synergistic actions of binding sites located on CH2, CH3, and the consensus binding site (CBS) of the Fc fragment. At acidic pH, the electrostatic repulsion between the basic residues and the protonated pyridine ring group on MEP ligands is the main driving force for the detachment of the Fc fragment from the MEP net.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1512, 25 August 2017, Pages 34-42
نویسندگان
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