Preliminary results for interval feeding the orthogonal pressurized planar electrochromatography system with sample solution for its preparative separation

- Two simultaneous and orthogonal processes of chromatography and electrophoresis/electrochromatography are applied to demonstrate further possibilities of the orthogonal pressurized planar electrochromatography (OPPEC) technique.
- Two-dimensionality of preparative mode of OPPEC technique is proved.
- The OPPEC technique allows on the separation of substances with the same electrophoretic mobility.
- The preparative OPPEC separation of such substances is based on the principle of frontal chromatography.
- In order to increase the separation efficiency the interval delivery of sample mixture to the separation system can be used.

The orthogonal pressurized planar electrochromatography (OPPEC) is an example of 2-D separation technique, in which two simultaneous and orthogonal processes of electrophoresis and chromatography are involved in the separation mechanism. In the case of preparative separation of substances characterized by different electrophoretic mobility, such separation system can be constantly fed with the sample solution and the separated components can be constantly collected at its outlet.In the paper, as opposed to the previous studies, we discuss the capabilities of OPPEC technique for preparative separation of substances characterized by the same electrophoretic mobility. According to the proposed solution, the separation system can be periodically fed with the sample solution and separated components can be collected alternately at its outlet. The advantages of this new approach over the column chromatography with regard to the separation of complex mixtures have been signaled.