کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5135434 1493453 2016 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Oxysterols in cosmetics-Determination by planar solid phase extraction and gas chromatography-mass spectrometry
ترجمه فارسی عنوان
اکسسترل در لوازم آرایشی-تعیین توسط استخراج فاز جامد فاز و اسپکترومتری جرم کروماتوگرافی گاز
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


- We developed a pSPE-GC-MS method for the analysis of sterol oxidation products.
- The method enabled the simultaneous determination of sterol oxide levels from animal and plant origin in cosmetic products.
- Structural elucidation of several unknown lanosterol and dihydrolanosterol oxidation products in lanolin containing products was performed by several analytical techniques.

Sterol oxidation products (SOPs) are linked to several toxicological effects. Therefore, investigation of potential dietary uptake sources particularly food of animal origin has been a key issue for these compounds. For the simultaneous determination of oxysterols from cholesterol, phytosterols, dihydrolanosterol and lanosterol in complex cosmetic matrices, planar solid phase extraction (pSPE) was applied as clean-up tool. SOPs were first separated from more non-polar and polar matrix constituents by normal phase thin-layer chromatography and then focussed into one target zone. Zone extraction was performed with the TLC-MS interface, followed by gas chromatography-mass spectrometry analysis. pSPE showed to be effective for cleaning up cosmetic samples as sample extracts were free of interferences, and gas chromatographic columns did not show any signs of overloading. Recoveries were between 86 and 113% with relative standard deviations of below 10% (n = 6). Results of our market survey in 2016 showed that some cosmetics with ingredients of plant origin contained phytosterol oxidation products (POPs) in the low ppm range and therefore in line with levels reported for food. In lanolin containing products, total SOPs levels (cholesterol oxidation products (COPs), lanosterol oxidation products (LOPs), dihydrolanosterol oxidation products (DOPs)) being in the low percent range exceeded reported levels for food by several orders of magnitudes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1473, 18 November 2016, Pages 10-18
نویسندگان
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