کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5135555 | 1493429 | 2017 | 8 صفحه PDF | دانلود رایگان |
- Novel one step in-situ functionalized monolithic polysiloxane-polymethacrylate composite material.
- Functionalization and crosslinking by double thiol-ene click-type polymerization reaction.
- Poly(3-mercaptopropyl)methylsiloxane as thiol component quantitatively incorporated.
- In-situ functionalization with O-tert-butylcarbamoylquinine.
- Nano-HPLC enantiomer separation of N-3,5-dinitrobenzoylleucine on chiral monolithic capillary column.
This work reports on the proof-of-principle of preparation of novel one step in-situ functionalized monolithic polysiloxane-polymethacrylate composite materials in capillary columns for enantioselective nano-HPLC using a thiol-ene click reaction. Quinine carbamate as functional monomer and ethylene dimethacrylate as crosslinker were both used as ene components in a thermally initiated double click-type polymerization reaction with poly(3-mercaptopropyl)methylsiloxane as thiol component in presence of 1-propanol as porogenic solvent. Elemental analysis and on-capillary fluorescence measurement proved the successful incorporation of the functional chiral monomer into the polymer. Scanning electron microscopy images revealed a macroporous polymer morphology which is typical for a nucleation and growth mechanism of pore formation. The individual microglobules appear relatively spherical and smooth indicating a non-porous nature. Nano-HPLC experiments of the chiral monolithic capillary column provided successful enantiomer separation of N-3,5-dinitrobenzoylleucine as test compound in polar organic elution mode clearly documenting the successful implementation of the proposed concept towards new functionalized monolithic composite materials.
Journal: Journal of Chromatography A - Volume 1497, 12 May 2017, Pages 172-179