کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5135818 1493455 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Evaluation of affinity-based serum clean-up in mass spectrometric analysis: Plastic vs monoclonal antibodies
ترجمه فارسی عنوان
بررسی اثر تمیز کردن سرم وابستگی در تجزیه و تحلیل طیف سنج جرمی: پلاستیک و آنتیبادیهای منوکلونال پلاسما
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


- Direct comparison of monoclonal and plastic antibodies.
- Evaluation of sample preparation of biological matrices.
- Identification of analytical interferences from serum.

Mass spectrometric assays are now of great relevance for trace compound analysis in complex matrices such as serum and plasma samples. Especially in the quantification of low abundant protein-biomarkers, the choice of the sample preparation is crucial. In the present paper immunocapture and Molecular Imprinted Polymers (MIPs) have been applied in the determination of pro-gastrin-releasing peptide, a Small Cell Lung Cancer marker. These affinity-based techniques were compared in terms of matrix effect, limits of detection, repeatability and extraction specificity. In addition, protein precipitation was included for comparison as it is a typical sample preparation method of biological matrices. The results highlighted differences in the methods' performance and specificity, strongly affecting the outcome of the mass spectrometric determination. Plastic and monoclonal antibodies confirmed to be sensitive and specific sample preparations able to determine ProGRP at clinical relevant concentration, although only the use of monoclonal antibodies allowed the reliable quantification of ProGRP at reference levels (8 pM). In addition better insight in the specificity of the three sample preparation techniques was gained. This might also be of interest for other biological applications.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1471, 4 November 2016, Pages 19-26
نویسندگان
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