کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5135864 | 1493458 | 2016 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Magnetization of 3-dimentional homochiral metal-organic frameworks for efficient and highly selective capture of phosphopeptides
ترجمه فارسی عنوان
مغناطیسی چارچوب های فلزی ارگانیک هوموکیال سه بعدی برای جذب فسفوپتید های مؤثر و بسیار انتخابی
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Enrichment of phosphopeptides based on various affinity probes prior to mass spectrometry detection is usually required due to the low abundance and low ionization efficiency of phosphopeptides. In this work, a 3-dimentional homochiral metal-organic frameworks (MOFs) was modified with magnetic nanoparticles using a facile method and then utilized for phosphopeptides capture with high efficiency and specificity. Based on magnetic solid phase extraction, a rapid and efficient method was developed and the whole enrichment procedure could be easily finished within 10Â min. Efficient and highly selective capture of phosphopeptides from tryptic digests and human serum was achieved. This affinity probe showed satisfactory reproducibility of the particle synthesis and could be recycled for at least seven times. With all the advantages mentioned above, this strategy is of great potential for routine application in phosphoproteomes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1468, 14 October 2016, Pages 49-54
Journal: Journal of Chromatography A - Volume 1468, 14 October 2016, Pages 49-54
نویسندگان
Xiaoyue Qi, Cuilan Chang, Xinyuan Xu, Yiding Zhang, Yu Bai, Huwei Liu,