کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5136160 1494002 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Enantiomeric separation and quantification of citalopram in serum by ultra-high performance supercritical fluid chromatography-tandem mass spectrometry
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Enantiomeric separation and quantification of citalopram in serum by ultra-high performance supercritical fluid chromatography-tandem mass spectrometry
چکیده انگلیسی


- A high throughput UHPSFC-MS/MS method for enantiomeric separation and quantification of R/S-citalopram in serum was developed and validated.
- The method provides efficient sample preparation with acidic acetonitrile and filtration through a phospholipid removal plate.
- The method has proved to be simple and robust for separation and quantification of R/S-citalopram in routine use.

A method for enantiomeric separation and quantification of R/S-citalopram in serum was developed and validated using ultra-high performance supercritical fluid chromatography-tandem mass spectrometry (UHPSFC-MS/MS). Sample preparation prior to UHPSFC-MS/MS analysis consisted of protein precipitation with acidic acetonitrile and filtration through a phospholipid removal plate. The UHPSFC-MS/MS method used an UPC2 Trefoil CEL2 column with a mobile phase consisting of CO2 and methanol/acetonitrile (70:30, v/v) with 10 mM ammonium acetate. The injection volume was 1 μL and run time was 4 min. MS/MS detection was performed with positive electrospray ionization and two multiple reaction monitoring transitions (m/z 325.1 > 262.0 and m/z 325.1 > 109.0). The calibration range was 5-500 nM for each analyte. The between-assay relative standard deviations were in the range of 3.4-4.5%. Recovery was 81-91% and matrix effects ranged from 96 to 101% (corrected with internal standard). After development and initial testing, the method has been successfully implemented in routine use in our laboratory for both separation and quantification of R/S-citalopram in more than 250 serum samples for therapeutic drug monitoring.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volumes 1061–1062, 1 September 2017, Pages 103-109
نویسندگان
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