کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5136228 1494001 2017 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Quantification of menadione from plasma and urine by a novel cysteamine-derivatization based UPLC-MS/MS method
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Quantification of menadione from plasma and urine by a novel cysteamine-derivatization based UPLC-MS/MS method
چکیده انگلیسی


- Menadione was quantified by UPLC-MS/MS after a new non-toxic and applicable cysteamine-derivatizating process.
- The MS detection was realized under positive mode after the stable isotope dilution.
- Benefitting from the excellent sensitivity and reliability of this method, the urinary free menadione was quantified for the very first time.

Menadione, as the crucial component of vitamin Ks, possessed significant nutritional and clinical values. However, there was still lack of favourable quantification strategies for it to date. For improvement, a novel cysteamine derivatization based UPLC-MS/MS method was presented in this work. The derivatizating reaction was proved non-toxic, easy-handling and high-efficient, which realized the MS detection of menadione under positive mode. Benefitting from the excellent sensitivity of the derivatizating product as well as the introduction of the stable isotope dilution technique, the quantification could be achieved in the range of 0.05-50.0 ng/mL for plasma and urine matrixes with satisfied accuracy and precision. After analysis of the samples from healthy volunteers after oral administration of menadione sodium bisulfite tablets, the urinary free menadione was quantified for the very first time. We believe the progress in this work could largely promote the exploration of the metabolic mechanism of vitamin K in vivo.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 1063, 15 September 2017, Pages 107-111
نویسندگان
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