کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5136666 | 1494021 | 2016 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
LC-MS/MS method for quantitation of seven biomarkers in human plasma for the assessment of insulin resistance and impaired glucose tolerance
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کلمات کلیدی
IGTLLOQPFPA3-hydroxybutyrateUHPLCMRMBioanalyticalFPG3-HBLC–MS/MS - LC-MS / MSimpaired glucose tolerance - اختلال تحمل گلوکزValidation - اعتبار سنجیFasting plasma glucose - قند خون ناشتاlower limit of quantitation - محدودیت پایین کمیتInsulin resistance - مقاومت به انسولینBiomarkers - نشانگر زیستی یا بیومارکرmultiple reaction monitoring - نظارت چندگانه چندگانهWis - وایUltra High Performance Liquid Chromatography - کروماتوگرافی مایع با کارایی فوق العاده بالاquality control - کنترل کیفیت
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Early detection of insulin resistance (IR) and/or impaired glucose tolerance (IGT) is crucial for delaying and preventing the progression toward type 2 diabetes. We recently developed and validated a straightforward metabolite-based test for the assessment of IR and IGT in a single LC-MS/MS method. Plasma samples were diluted with isotopically-labeled internal standards and extracted by simple protein precipitation. The extracts were analyzed by LC-MS/MS for the quantitation of 2-hydroxybutyric acid (0.500-40.0 μg/mL), 3-hydroxybutyric acid (1.00-80.0 μg/mL), 4-methyl-2-oxopentanoic acid (0.500-20.0 μg/mL), 1-linoleoyl-2-hydroxy-sn-glycero-3-phosphocholine (2.50-100 μg/mL), oleic acid (10.0-400 μg/mL), pantothenic acid (0.0100-0.800 μg/mL), and serine (2.50-100 μg/mL). Liquid chromatography was carried out on a reversed phase column with a run time of 3.1 min and the mass spectrometer operated in negative MRM mode. Method validation was performed on three identical LC-MS/MS systems with five runs each. Sufficient linearity (R2 > 0.99) was observed for all the analytes over the ranges. The imprecision (CVs) was found to be less than 5.5% for intra-run and less than 5.8% for inter-run for the seven analytes. The analytical recovery was determined to be between 96.3 and 103% for the seven analytes. This fast and robust method has subsequently been used for patient sample analysis for the assessment of IR and IGT.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 1038, 1 December 2016, Pages 101-108
Journal: Journal of Chromatography B - Volume 1038, 1 December 2016, Pages 101-108
نویسندگان
Qibo Zhang, Lisa A. Ford, Kelli D. Goodman, Tiffany A. Freed, Deirdre M. Hauser, Jessie K. Conner, Kate E.T. Vroom, Douglas R. Toal,